pCool-hDDB1-BPB
(Plasmid
#48639)
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PurposeExpresses DDB1 BPB domain (aa 391-709) in Ecoli (ampR)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48639 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCool
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Backbone manufacturerpGex2T
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehDDB1-BPB
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SpeciesH. sapiens (human)
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GenBank IDref|NP_001914.3|
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Entrez GeneDDB1 (a.k.a. DDBA, UV-DDB1, WHIKERS, XAP1, XPCE, XPE, XPE-BF)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
BL21 is recommended for protein overexpression.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCool-hDDB1-BPB was a gift from Ning Zheng (Addgene plasmid # 48639 ; http://n2t.net/addgene:48639 ; RRID:Addgene_48639) -
For your References section:
Structure of DDB1 in complex with a paramyxovirus V protein: viral hijack of a propeller cluster in ubiquitin ligase. Li T, Chen X, Garbutt KC, Zhou P, Zheng N. Cell. 2006 Jan 13;124(1):105-17. 10.1016/j.cell.2005.10.033 PubMed 16413485