PurposeMammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48674||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.3 TOPO
- Total vector size (bp) 9739
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert nameCas9-VP64, nuclease-null
Insert Size (bp)4300
- Promoter CMV
/ Fusion Proteins
- NLS (C terminal on insert)
- VP64 (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer TK-pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M-SPn-VP64 was a gift from George Church (Addgene plasmid # 48674 ; http://n2t.net/addgene:48674 ; RRID:Addgene_48674)
For your References section:Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681. 10.1038/nmeth.2681 PubMed 24076762