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pGGY001
(Plasmid #48866)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 48866 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGreenII
  • Backbone size (bp) 2492
  • Vector type
    Golden Gate compatible plant transformation vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Gentamicin, 25 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Growth instructions
    This plasmid should be grown at reduced gentamycin concentration (5 µg/mL).
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is designed as destination vector for Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using KpnI and EcoRI. The 3' BsaI overhang can be exchanged using XhoI and XbaI. There is also a a NotI-PacI-SgsI-SfaAI-KpnI MCS in front of the A-overhang and an XbaI-PaeI MCS after the G-overhang.

Please note that a transposon insertion is present in the chloramphenicol resistance promoter. This insertion does not interfere with plasmid function. There is an additional BsaI site at bp# 1889-1894 which is irrelevant for GreenGate reactions with this plasmid.

Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 640-650bp
transposon integration = 747-2077bp
BsaI site #2 = 3414-3419bp

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGGY001 was a gift from Jan Lohmann (Addgene plasmid # 48866 ; http://n2t.net/addgene:48866 ; RRID:Addgene_48866)
  • For your References section:

    GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J. PLoS One. 2013 Dec 20;8(12):e83043. doi: 10.1371/journal.pone.0083043. PubMed 24376629
Included in kit:
Commonly requested with: