PurposeGateway adapted transfer of Neomycin resistance gene
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48993||Standard format: Plasmid sent in bacteria as agar stab||1||$85 *|
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Backbone manufacturerLife Technologies (Invitrogen)
- Backbone size w/o insert (bp) 2500
- Total vector size (bp) 3300
Vector typeGateway attB1 & attB2
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameaminoglycoside 3' phosphotransferase
Alt nameNeomycin resistance gene
Insert Size (bp)804
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 for
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
Please note that Addgene's sequencing results found a few differences when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing laboratory, these differences are not a concern for the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDONR221-Neo was a gift from Rick Tarleton (Addgene plasmid # 48993 ; http://n2t.net/addgene:48993 ; RRID:Addgene_48993)
For your References section:Evaluation of high efficiency gene knockout strategies for Trypanosoma cruzi. Xu D, Brandan CP, Basombrio MA, Tarleton RL. BMC Microbiol. 2009 May 11;9:90. doi: 10.1186/1471-2180-9-90. 10.1186/1471-2180-9-90 PubMed 19432966