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GABA(A) receptor subunit B3SE
(Plasmid #49171)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49171 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 49171-D50 50 μg of DNA in Tris buffer $495
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DNA 49171-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    modified pRK5
  • Backbone manufacturer
    BD PharMingen
  • Total vector size (bp) 7097
  • Modifications to backbone
    region 5’ of the cloning site is slightly different
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GABA(A) receptor subunit beta 3
  • Alt name
    Gabrb3
  • Species
    M. musculus (mouse)
  • Mutation
    GTG (V) to TGC (C)
  • GenBank ID
  • Entrez Gene
    Gabrb3 (a.k.a. A230092K12Rik, Cp1, Gabrb-3, beta3)
  • Promoter CMV
  • Tags / Fusion Proteins
    • pHGFP (pHluorin) (N terminal on insert)
    • myc (N terminal on insert)
    • thrombin cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer P5
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Deletion of YK at end of pHGFP, added extra base to keep frame before myc-tag

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GABA(A) receptor subunit B3SE was a gift from Tija Jacob & Stephen Moss (Addgene plasmid # 49171 ; http://n2t.net/addgene:49171 ; RRID:Addgene_49171)

  • For your References section:

    Gephyrin regulates the cell surface dynamics of synaptic GABAA receptors. Jacob TC, Bogdanov YD, Magnus C, Saliba RS, Kittler JT, Haydon PG, Moss SJ. J Neurosci. 2005 Nov 9;25(45):10469-78. 10.1523/JNEUROSCI.2267-05.2005 PubMed 16280585
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