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pJFA334E9
(Plasmid #49237)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49237 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    unknown
  • Backbone manufacturer
    Joung lab
  • Vector type
    TALEN ; TALE-TET1 Expression Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TET1 tet methylcytosine dioxygenase 1
  • Species
    H. sapiens (human), Synthetic
  • Entrez Gene
    TET1 (a.k.a. CXXC6, LCX, bA119F7.1)
  • Promoter EF1a

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Location of plasmid features:
EF1alpha promoter = 922-2106
NLS = 2118-2142
TALE N-terminal domain = 2167-2577
Clone TALE array repeats between BsmBI sites
TALE C-terminal domain = 2665-2949
TET1 catalytic domain = 2956-5123
BGH polyA = 6369-6596

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJFA334E9 was a gift from Keith Joung (Addgene plasmid # 49237 ; http://n2t.net/addgene:49237 ; RRID:Addgene_49237)
  • For your References section:

    Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins. Maeder ML, Angstman JF, Richardson ME, Linder SJ, Cascio VM, Tsai SQ, Ho QH, Sander JD, Reyon D, Bernstein BE, Costello JF, Wilkinson MF, Joung JK. Nat Biotechnol. 2013 Oct 9. doi: 10.1038/nbt.2726. 10.1038/nbt.2726 PubMed 24108092