Purposeexpresses human p53 deltaN linked to full length p53 with C terminal His tag and also expresses EGFP in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49244||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5300
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberLow Copy
Gene/Insert namep53 deltaN and p53 WT
SpeciesH. sapiens (human)
Insert Size (bp)2343
Entrez GeneTP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
- Promoter CMV
/ Fusion Proteins
- His (C terminal on insert)
- IRES-EGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Note there are some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequence. These differences are not known to affect function, though they may affect diagnostic digests.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIRES2-EGFP-deltaNp53:WTp53 was a gift from Dylan Taatjes (Addgene plasmid # 49244 ; http://n2t.net/addgene:49244 ; RRID:Addgene_49244)
For your References section:The human DeltaNp53 isoform triggers metabolic and gene expression changes that activate mTOR and alter mitochondrial function. Lin SC, Karoly ED, Taatjes DJ. Aging Cell. 2013 Oct;12(5):863-72. doi: 10.1111/acel.12108. Epub 2013 Jun 25. 10.1111/acel.12108 PubMed 23734707