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pLP-mOlig2-ires-gfp
(Plasmid #49399)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49399 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLP-IRES2-EGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression, Cre/Lox ; acceptor vector
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Olig2
  • Alt name
    oligodendrocyte transcription factor 2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1755
  • GenBank ID
    BC051967
  • Entrez Gene
    Olig2 (a.k.a. AI604895, Bhlhb, Bhlhb1, Olg, Olg-2, Oligo2, RK1, RK17, bHLHe1, bHLHe19)
  • Promoter CMV
  • Tags / Fusion Proteins
    • IRES (C terminal on backbone)
    • EGFP (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

mOlig2 was transferred from the donor vector (pDNR-mOlig2, Addgene plasmid #49397) to the acceptor vector using Cre-mediated recombination at the LoxP sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLP-mOlig2-ires-gfp was a gift from Randall McKinnon (Addgene plasmid # 49399 ; http://n2t.net/addgene:49399 ; RRID:Addgene_49399)