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pT7CFE1-TelR15-mTagBFP2
(Plasmid #49643)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49643 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pT7CFE1
  • Backbone manufacturer
    Pierce
  • Backbone size w/o insert (bp) 3600
  • Total vector size (bp) 6300
  • Vector type
    In vitro Translation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TelR15
  • Species
    Synthetic
  • Insert Size (bp)
    2000
  • Promoter T7
  • Tag / Fusion Protein
    • mTagBFP2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site SbfI (unknown if destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 Terminator
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7CFE1-TelR15-mTagBFP2 was a gift from Thoru Pederson (Addgene plasmid # 49643 ; http://n2t.net/addgene:49643 ; RRID:Addgene_49643)
  • For your References section:

    Visualization of repetitive DNA sequences in human chromosomes with transcription activator-like effectors. Ma H, Reyes-Gutierrez P, Pederson T. Proc Natl Acad Sci U S A. 2013 Dec 9. 10.1073/pnas.1319097110 PubMed 24324157