M13mp1
(Plasmid
#49971)
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Purpose(Empty Backbone) M13 cloning vector
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49971 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneM13
- Backbone size (bp) 7196
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Modifications to backbonelac fragment inserted into M13
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Vector typecloning vector
Growth in Bacteria
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Bacterial Resistance(s)None
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsPlate on X-gal and IPTG and select blue colonies.
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
M13mp1 was a gift from Joachim Messing (Addgene plasmid # 49971) -
For your References section:
Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro. Messing J, Gronenborn B, Muller-Hill B, Hans Hopschneider P. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3642-6. 10.1073/pnas.74.9.3642 PubMed 333444
