pENTR-p14FAST
(Plasmid #50025)

• Purpose: pENTR vector for reptilian orthoreovirus p14 fusion-associated small transmembrane (FAST) protein
• Depositing Lab: Jamie Davies
• Publication: Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollection 2014.

Backbone

• Vector backbone: pDONR221
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 2549
• Total vector size (bp): 2927
• Vector type: Entry vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p14FAST
• Alt name: p14 fusion-associated small transmembrane (FAST) protein
• Species: Reptilian orthoreovirus
• Insert Size (bp): 378
• GenBank ID: AY238887

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13_F
• 3′ sequencing primer: M13_R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

When p14FAST is expressed in mammalian cells, fusion takes place between expressing cells and any other neighbouring cells that are in contact (even with non-expressing cells of a different cell type). Potential users should note that this implies a risk that cells carrying the fusion gene might fuse with cells of living humans, and potentially carry dangerous genetic material such as oncogenes and viruses into them (even basic cells lines carry mutations making them proliferative and immortal). Appropriate precautions should therefore be taken when handling the cultures.