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(Plasmid #50025)


Item Catalog # Description Quantity Price (USD)
Plasmid 50025 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 2549
  • Total vector size (bp) 2927
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    p14 fusion-associated small transmembrane (FAST) protein
  • Species
    Reptilian orthoreovirus
  • Insert Size (bp)
  • GenBank ID

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

When p14FAST is expressed in mammalian cells, fusion takes place between expressing cells and any other neighbouring cells that are in contact (even with non-expressing cells of a different cell type). Potential users should note that this implies a risk that cells carrying the fusion gene might fuse with cells of living humans, and potentially carry dangerous genetic material such as oncogenes and viruses into them (even basic cells lines carry mutations making them proliferative and immortal). Appropriate precautions should therefore be taken when handling the cultures.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-p14FAST was a gift from Jamie Davies (Addgene plasmid # 50025 ; ; RRID:Addgene_50025)
  • For your References section:

    A library of mammalian effector modules for synthetic morphology. Cachat E, Liu W, Hohenstein P, Davies JA. J Biol Eng. 2014 Nov 19;8(1):26. eCollection 2014. 158 [pii] PubMed 25478005