|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50353||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript II KS-
- Backbone size w/o insert (bp) 2961
- Total vector size (bp) 5609
Vector typeBacterial Expression
Growth in Bacteria
Alt nameargonaute 1
SpeciesM. musculus (mouse)
Entrez GeneEif2c1 (a.k.a. Ago1)
- Promoter T3
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Addgene sequencing has found a T736A mutation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBluescript-mouse eIF2C1 was a gift from Kumiko Ui-Tei (Addgene plasmid # 50353 ; http://n2t.net/addgene:50353 ; RRID:Addgene_50353)
For your References section:Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors. Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K. Curr Biol. 2003 Jan 8;13(1):41-6. 10.1016/S0960-9822(02)01394-5 PubMed 12526743