pCDNA CrkII Y221F
(Plasmid
#50732)
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Purposemammalian expression of CrkII Y221F mutant
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 50732 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCDNA 3.1
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCrkII Y221F
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Alt nameCrkII
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SpeciesH. sapiens (human)
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MutationY221F
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GenBank IDGI:1219554
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Entrez GeneCRK (a.k.a. CRKII, p38)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA CrkII Y221F was a gift from Kenneth Yamada (Addgene plasmid # 50732 ; http://n2t.net/addgene:50732 ; RRID:Addgene_50732) -
For your References section:
Tyrosine phosphorylation of the CrkII adaptor protein modulates cell migration. Takino T, Tamura M, Miyamori H, Araki M, Matsumoto K, Sato H, Yamada KM. J Cell Sci. 2003 Aug 1;116(Pt 15):3145-55. Epub 2003 Jun 10. 10.1242/jcs.00632 PubMed 12799422