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pscAluYNF1
(Plasmid #50934)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50934 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAlu-NeoTet
  • Backbone manufacturer
    Dewannieux,M., Esnault,C. and Heidmann,T. (2003) LINE-mediated retrotransposition of marked Alu sequences. Nat. Genet., 35, 41-4
  • Backbone size w/o insert (bp) 2773
  • Total vector size (bp) 2855
  • Modifications to backbone
    the neoTet reporter gene was removed
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    scAluYNF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    114
  • Mutation
    the poly(A) linker and the right arm of the dimeric ALuYNF1 gene were removed
  • Promoter 7SL RNA promoter (pol III)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Wallace, M.R. et al. A de novo Alu insertion results in neurofibromatosis type 1. Nature 353, 864–866 (1991).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pscAluYNF1 was a gift from Katharina Strub (Addgene plasmid # 50934 ; http://n2t.net/addgene:50934 ; RRID:Addgene_50934)
  • For your References section:

    Alu RNA regulates the cellular pool of active ribosomes by targeted delivery of SRP9/14 to 40S subunits. Ivanova E, Berger A, Scherrer A, Alkalaeva E, Strub K. Nucleic Acids Res. 2015 Mar 11;43(5):2874-87. doi: 10.1093/nar/gkv048. Epub 2015 Feb 19. 10.1093/nar/gkv048 PubMed 25697503