PurposeExpress GST-tagged hAIM2 in bacteria
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51528||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5443
- Total vector size (bp) 7119
Vector typeBacterial Expression
Growth in Bacteria
SpeciesH. sapiens (human)
Insert Size (bp)1029
Entrez GeneAIM2 (a.k.a. PYHIN4)
- Promoter lac operator
/ Fusion Protein
- GST (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer T7
- 3′ sequencing primer His6 (Common Sequencing Primers)
Terms and Licenses
For protein expression the plasmid needs to be transformed into BL21 E. coli.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET21a-hAIM2-GST was a gift from Emad Alnemri (Addgene plasmid # 51528 ; http://n2t.net/addgene:51528 ; RRID:Addgene_51528)
For your References section:AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA. Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES. Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub 2009 Jan 21. 10.1038/nature07710 PubMed 19158676