pET41-GST-KaiC
(Plasmid #68051)

• Purpose: Strain: AM5117, Used for protein purification
• Depositing Lab: Susan Golden
• Publication: Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17765-9. doi: 10.1073/pnas.1216401109. Epub 2012 Oct 15.

Backbone

• Vector backbone: pET41a(+)
• Total vector size (bp): 7500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: KaiC
• Species: Synechococcus elongatus
• Insert Size (bp): 1500
• GenBank ID: CP000100
• Promoter: T7
• Tags / Fusion Proteins:
  • GST (N terminal on backbone)
  • 6xHis (N terminal on backbone)
  • S-Tag

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pGEX5
• 3′ sequencing primer: T7term

Resource Information

• Supplemental Documents:
  • Strain Database Information

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PreScission (GE Healthcare) Protease cutting site is introduced before KaiC. KaiC is cloned between NcoI and HiNdII. Use BL21DE3 and GST column for protein purification. Use PreScission Protease to cut GST tag off.