PurposeStrain: AM5116, Used for protein purification
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||68050||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Total vector size (bp) 6300
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Insert Size (bp)300
- Promoter T7
/ Fusion Proteins
- 6xHis (N terminal on backbone)
- SUMO (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Ulp1 cutting site introduced before KaiB. Sumo-KaiB cloned between NdeI and HindIII. No enterokinase cutting site. Use BL21DE3 and Ni column for protein purification.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET28-SUMO-KaiB was a gift from Susan Golden (Addgene plasmid # 68050 ; http://n2t.net/addgene:68050 ; RRID:Addgene_68050)
For your References section:Oxidized quinones signal onset of darkness directly to the cyanobacterial circadian oscillator. Kim YI, Vinyard DJ, Ananyev GM, Dismukes GC, Golden SS. Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17765-9. doi: 10.1073/pnas.1216401109. Epub 2012 Oct 15. 10.1073/pnas.1216401109 PubMed 23071342