M4786 KanMX::hisG-URA3-HisG Disruptor Converter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51690||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneM4757 (kanMX::TRP1), Addgene plasmid #51687
Vector typeYeast Expression ; yeast marker swap
Growth in Bacteria
SpeciesS. cerevisiae (budding yeast)
Entrez GeneURA3 (a.k.a. YEL021W)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
M4786 (kanMX::hisG-URA3-hisG) was made by digesting M4757 with BamHI and SalI and inserting a 3.9 kb BamHI–SalI fragment with hisG–URA3–hisG from plasmid M2524.
The kanMX::hisG–URA3–hisG converter is recyclable, so that any MX-based disruption can be reverted to an unmarked disruption, allowing reuse of markers.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M4786 KanMX::hisG-URA3-HisG Disruptor Converter was a gift from David Stillman (Addgene plasmid # 51690 ; http://n2t.net/addgene:51690 ; RRID:Addgene_51690)
For your References section:New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Voth WP, Jiang YW, Stillman DJ. Yeast. 2003 Aug;20(11):985-93. 10.1002/yea.1018 PubMed 12898713