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(Plasmid #51711)


Item Catalog # Description Quantity Price (USD)
Plasmid 51711 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pC-PTP-NEO (Addgene plasmid #51710)
  • Modifications to backbone
    The resistance marker cassette was modified by replacing NEO-R by the coding region of the puromycin N-acetyltransferase gene (PURO-R).
  • Vector type
    T. brucei genome integration vectors
  • Selectable markers
  • Tag / Fusion Protein
    • PTP (ProtC-TEV-ProtA) (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The PTP tag is a derivative of the original TAP tag and has CBP replaced by ProtC.

The PTP cassette is composed of 434 bp of 5′ flank from the TbRPA2 gene, encoding the second-largest subunit of RNA polymerase I, a translation initiation codon, the PTP tag coding sequence, and unique restriction sites for fusing target sequences to the tag.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pN-PURO-PTP was a gift from Arthur Gunzl (Addgene plasmid # 51711 ; ; RRID:Addgene_51711)
  • For your References section:

    Highly efficient tandem affinity purification of trypanosome protein complexes based on a novel epitope combination. Schimanski B, Nguyen TN, Gunzl A. Eukaryot Cell. 2005 Nov;4(11):1942-50. 10.1128/EC.4.11.1942-1950.2005 PubMed 16278461