|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51873||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
- Total vector size (bp) 12100
Modifications to backboneDigested with EcoRI, ligated with an adaptor containing a NotI site and then digested with NotI
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesR. norvegicus (rat)
Insert Size (bp)7400
Entrez GeneHivep2 (a.k.a. MIBP1)
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer GGTTCGGCTTCTGGCGTGTG
- 3′ sequencing primer AGGGCATTGGCCACACCAGC (Common Sequencing Primers)
Terms and Licenses
Makino, R., Akiyama, K., Yasuda, J., Mashiyama, S., Honda, S., Sekiya, T., & Hayashi, K. (1994). Cloning and characterization of a c-myc intron binding protein (MIBP1). Nucleic acids research, 22(25), 5679-5685.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAE-MIBP1 was a gift from Kenshi Hayashi & Tomoko Tahira (Addgene plasmid # 51873 ; http://n2t.net/addgene:51873 ; RRID:Addgene_51873)
For your References section:Characterization of the biological functions of a transcription factor, c-myc intron binding protein 1 (MIBP1). Fukuda S, Yamasaki Y, Iwaki T, Kawasaki H, Akieda S, Fukuchi N, Tahira T, Hayashi K. J Biochem. 2002 Mar;131(3):349-57. 10.1093/oxfordjournals.jbchem.a003109 PubMed 11872163