mSECFP/pcDNA3
(Plasmid #51951)

• Purpose: mammalian expression of mSECFP, a monomeric super enhanced cyan fluorescent protein
• Depositing Lab: Takeharu Nagai
• Publication: Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi: 10.1038/nmeth.1193. Epub 2008 Mar 16.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mSECFP
• Species: Synthetic
• Insert Size (bp): 700

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGHrev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GenBank accession number: AB435576. ECFP was modified to improve its brightness and oligomeric property by substituting Serine at 72 for Alanine, Serine at 175 for Glysine, and Alanine at 206 for Lysine, yielding mseCFP.