PurposeExpresses full-length Fibrinogen gamma chain precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||52031||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerYves Durocher, NRC
- Backbone size w/o insert (bp) 6618
- Total vector size (bp) 8000
Modifications to backboneStop codon at amino acid 454, no translation of rat CD4 d3+4, enzymatic biotinylation sequence, linker and His tag (CD4d3+4-BioLHis).
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameFibrinogen gamma chain precursor
SpeciesH. sapiens (human)
Insert Size (bp)1382
MutationStop Codon at amino acid 454, before CD4, bio and His tags
- Promoter CMV
/ Fusion Proteins
- rat CD4 d3+4 (C terminal on backbone)
- enzymatic biotinylation sequence (C terminal on backbone)
- His tag (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer CMV-fwd (Common Sequencing Primers)
Article ref 83
Co-transfect with FGA-bio-His (Addgene 52029) and FGB (Addgene 52030) to create the fibrinogen trimer
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FGG was a gift from Gavin Wright (Addgene plasmid # 52031 ; http://n2t.net/addgene:52031 ; RRID:Addgene_52031)
For your References section:A human platelet receptor protein microarray identifies FcepsilonR1alpha as an activating PEAR1 ligand. Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright GJ. Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946. 10.1074/mcp.M114.046946 PubMed 25713122