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pET28-GG-PUF
(Plasmid #52137)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52137 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size (bp) 5930
  • Modifications to backbone
    Insert containing PUM-HD flanking regions 1' and 8', separated by LacZ alpha, cloned using the Gibson Assembly method.
  • Vector type
    Bacterial Expression
  • Promoter T7
  • Tags / Fusion Proteins
    • PUM-HD flanking region 1' (N terminal on insert)
    • PUM-HD flanking region 8' (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-GG-PUF was a gift from Huimin Zhao (Addgene plasmid # 52137 ; http://n2t.net/addgene:52137 ; RRID:Addgene_52137)
  • For your References section:

    Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. Abil Z, Denard CA, Zhao H. J Biol Eng. 2014 Mar 1;8(1):7. 10.1186/1754-1611-8-7 PubMed 24581042