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PSD-95_Y12E-pTagRFP
(Plasmid #52672)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52672 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTagRFP
  • Backbone manufacturer
    Evrogen
  • Backbone size w/o insert (bp) 4733
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PSD-95
  • Alt name
    Postsynaptic density protein 95
  • Alt name
    Disks large homolog 4 (DLG4)
  • Alt name
    Synapse-associated protein 90 (SAP-90)
  • Species
    R. norvegicus (rat)
  • Mutation
    Y12E
  • GenBank ID
    P78352
  • Entrez Gene
    Dlg4 (a.k.a. Dlgh4, PSD95, Sap90)
  • Promoter CMV
  • Tag / Fusion Protein
    • tagRFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer mKate2-TurboRFP-R
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HindII and EcoRI were used to cut PSD-95 from PSD-95-pGW1 (received from David Bredt) and ligate it into pTagRFP-N. PSD-95 tyrosine 12 was mutated to glutamate (Y12E) by extending a complementary set of primers (PSD95Y12E-forward: 5’-GAC AAC CAA GAA AGA GCG CTA CCA AGA TGA AGA CAC G-3’, PSD95Y12E-reverse: 5'-CGT GTC TTC ATC TTG GTA GCG CTC TTT CTT GGT TGT C-3') along the whole template plasmid using Q5 high-fidelity DNA polymerase (NEB). DpnI was subsequently used to digest non-mutated, methylated template DNA prior to transformation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PSD-95_Y12E-pTagRFP was a gift from Johannes Hell (Addgene plasmid # 52672 ; http://n2t.net/addgene:52672 ; RRID:Addgene_52672)