PurposeExpression of Kss1 in bacteria
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||52682||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5400
Vector typeBacterial Expression
Growth in Bacteria
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)1100
Entrez GeneKSS1 (a.k.a. YGR040W)
- Promoter T7
/ Fusion Proteins
- His6 (N terminal on backbone)
- T7 tag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
When co-expressed with Ste7EE (addgene plasmid #52681), produces active phosphorylated Kss1.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET28-Kss1 was a gift from Benjamin Turk (Addgene plasmid # 52682 ; http://n2t.net/addgene:52682 ; RRID:Addgene_52682)
For your References section:Deciphering protein kinase specificity through large-scale analysis of yeast phosphorylation site motifs. Mok J, Kim PM, Lam HY, Piccirillo S, Zhou X, Jeschke GR, Sheridan DL, Parker SA, Desai V, Jwa M, Cameroni E, Niu H, Good M, Remenyi A, Ma JL, Sheu YJ, Sassi HE, Sopko R, Chan CS, De Virgilio C, Hollingsworth NM, Lim WA, Stern DF, Stillman B, Andrews BJ, Gerstein MB, Snyder M, Turk BE. Sci Signal. 2010 Feb 16;3(109):ra12. doi: 10.1126/scisignal.2000482. 10.1126/scisignal.2000482 PubMed 20159853