Purposeexpression of constitutively active Ste7 kinase in bacteria
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||52681||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerGE Healthcare Life Sciences
- Backbone size w/o insert (bp) 4900
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)1545
MutationS359E/T363E constitutively active
Entrez GeneSTE7 (a.k.a. YDL159W)
- Promoter tac
/ Fusion Proteins
- GST (N terminal on backbone)
- Myc (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
A F2V mutation was discovered, it should not effect the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX4T2-Ste7-EE was a gift from Benjamin Turk (Addgene plasmid # 52681 ; http://n2t.net/addgene:52681 ; RRID:Addgene_52681)
For your References section:Deciphering protein kinase specificity through large-scale analysis of yeast phosphorylation site motifs. Mok J, Kim PM, Lam HY, Piccirillo S, Zhou X, Jeschke GR, Sheridan DL, Parker SA, Desai V, Jwa M, Cameroni E, Niu H, Good M, Remenyi A, Ma JL, Sheu YJ, Sassi HE, Sopko R, Chan CS, De Virgilio C, Hollingsworth NM, Lim WA, Stern DF, Stillman B, Andrews BJ, Gerstein MB, Snyder M, Turk BE. Sci Signal. 2010 Feb 16;3(109):ra12. doi: 10.1126/scisignal.2000482. 10.1126/scisignal.2000482 PubMed 20159853