pFA6a-kanMX6-PGAL1-GFP (F64L, S65T, V163A)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53205||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast genomic targeting
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameGAL1 promoter
SpeciesS. cerevisiae (budding yeast)
Entrez GeneGAL1 (a.k.a. YBR020W)
- Promoter Gal1 promoter
/ Fusion Proteins
- kanMX6 (N terminal on insert)
- GFP (F64L, S65T, V163A) (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
GFP in Addgene plasmid #41614 was replaced with stable GFP variant (F64L, S65T, V163A).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFA6a-kanMX6-PGAL1-GFP (F64L, S65T, V163A) was a gift from John Pringle (Addgene plasmid # 53205 ; http://n2t.net/addgene:53205 ; RRID:Addgene_53205)