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HSV-ENK-GFP
(Plasmid #53328)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53328 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pHSV-PrPUC
  • Backbone manufacturer
    Dr. Rachael Neve, McLean Hospital, Boston, MA
  • Backbone size w/o insert (bp) 5307
  • Total vector size (bp) 8692
  • Modifications to backbone
    Addition of GFP (747bp).
  • Vector type
    Mammalian Expression ; HSV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    enkephalin
  • Alt name
    ENK
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2637
  • Mutation
    contains promoter, -2609 to +52
  • Entrez Gene
    Penk (a.k.a. Enk, Penk-rs, Penk1, Penk2)
  • Promoter enkephalin
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HSV-ENK-GFP was a gift from John Neumaier (Addgene plasmid # 53328)

  • For your References section:

    Transient neuronal inhibition reveals opposing roles of indirect and direct pathways in sensitization. Ferguson SM, Eskenazi D, Ishikawa M, Wanat MJ, Phillips PE, Dong Y, Roth BL, Neumaier JF. Nat Neurosci. 2011 Jan;14(1):22-4. doi: 10.1038/nn.2703. Epub 2010 Dec 5. 10.1038/nn.2703 PubMed 21131952
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