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(Plasmid #53574)


Item Catalog # Description Quantity Price (USD)
Plasmid 53574 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pBad/His B
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4092
  • Total vector size (bp) 5111
  • Modifications to backbone
    The sequence ccATGgcagc was added to the Adonis aestivalis cbfd2 cDNA (AdKeto2) by PCR in order to provide first three codons that were missing from this cDNA, and also to introduce an NcoI site at the initiation codon. A 1.12 kb NcoI-XhoI fragment (with the XhoI site provided by the C terminal adaptor used in construction of the cDNA library) was then cloned in-frame in the NcoI and XhoI sites of pBAD/His B.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
    carotenoid β-ring 4-dehydrogenase
  • Species
    Adonis aestivalis
  • Insert Size (bp)
  • GenBank ID
  • Promoter araBAD

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCBFD2Bad was a gift from Francis X Cunningham Jr (Addgene plasmid # 53574 ; ; RRID:Addgene_53574)
  • For your References section:

    Elucidation of the pathway to astaxanthin in the flowers of Adonis aestivalis. Cunningham FX Jr, Gantt E. Plant Cell. 2011 Aug;23(8):3055-69. doi: 10.1105/tpc.111.086827. Epub 2011 Aug 23. 10.1105/tpc.111.086827 PubMed 21862704