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(Plasmid #53364)


Item Catalog # Description Quantity Price (USD)
Plasmid 53364 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Francis X. Cunningham, Jr.
  • Backbone size w/o insert (bp) 5271
  • Total vector size (bp) 6707
  • Modifications to backbone
    To make pCBFD1/HBFD1Bad, the cbfd1 cDNA was excised from pCBFD1 as a PvuII-XhoI fragment and ligated into pHBFD1Bad that had been digested with KpnI, blunted with the Klenow enzyme, purified by phenol/chloroform extraction, and then digested with XhoI and gel purified.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Growth instructions
    For production of astaxanthin, introduce this plasmid into E. coli strain TOP10 that already contains plasmid pAC-BETA or pAC-BETAipi, induce expression of the hbfd1 cDNA with arabinose (0.1% is satisfactory), and grow in liquid culture on a platform shaker at 28 degrees Celsius in darkness for 2-3 days.
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
    carotenoid β-ring 4-dehydrogenase
  • Species
    Adonis aestivalis
  • Insert Size (bp)
  • GenBank ID
  • Promoter lac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PvuII (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCBFD1/HBFD1Bad was a gift from Francis X Cunningham Jr (Addgene plasmid # 53364 ; ; RRID:Addgene_53364)
  • For your References section:

    Elucidation of the pathway to astaxanthin in the flowers of Adonis aestivalis. Cunningham FX Jr, Gantt E. Plant Cell. 2011 Aug;23(8):3055-69. doi: 10.1105/tpc.111.086827. Epub 2011 Aug 23. 10.1105/tpc.111.086827 PubMed 21862704