|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53635||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Growth Strain(s)Mach1 ; BL21(DE3)-R3-pRARE2
Copy numberHigh Copy
Insert Size (bp)891
MutationBacterial expression for structure determination; may not be full ORF, see comments
- Promoter T7
/ Fusion Protein
- His-6-SUMO (N terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
T7/lac regulated, N-terminal His-SUMO tags, SUMO protease, LIC cloning using BsaI digest/T4 polymerase but non-standard LIC sites, SacB stuffer fragment. Optional non-cleavable biotinylation and His6 tags if reverse primer does not encode stop codon. NB. SUMO recognition sequence is a dummy entry. http://www.thesgc.org/structures/4PTB/ Use BL21(DE3)-R3-pRARE2 strain for expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SP100A-c082 was a gift from Nicola Burgess-Brown (Addgene plasmid # 53635 ; http://n2t.net/addgene:53635 ; RRID:Addgene_53635)