PurposeLocalization: Focal Adhesions, Excitation: 399, Emission: 456
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||55334||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationV234L in Vinculin.
Entrez GeneVCL (a.k.a. CMD1W, CMH15, HEL114, MV, MVCL)
- Promoter CMV
/ Fusion Protein
- mTagBFP2 (N terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
. Excitation = 399; Emission = 456
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mTagBFP2-Vinculin-23 was a gift from Michael Davidson (Addgene plasmid # 55334 ; http://n2t.net/addgene:55334 ; RRID:Addgene_55334)
For your References section:An enhanced monomeric blue fluorescent protein with the high chemical stability of the chromophore. Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV. PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8. 10.1371/journal.pone.0028674 PubMed 22174863