pENTR-THT
(Plasmid #55790)

• Purpose: (Empty Backbone) GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Kanamycin resistance
• Depositing Lab: Stephan Geley
• Publication: Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi: 10.1371/journal.pone.0097764. eCollection 2014.

Backbone

• Vector backbone: pENTR-1A
• Backbone manufacturer: Invitrogen
• Backbone size (bp): 2500
• Modifications to backbone: introduction of TetO-flanked H1 promoter stuffer between BglII/HindIII
• Vector type: Mammalian Expression, RNAi ; GATEWAY
• Promoter: modified H1 RNA gene

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: 5'-CTGCAGGAATTCGAACGCTGACG-3' (ON1108)
• 3′ sequencing primer: 5'-TGTAAAACGACGGCCAGT-3' (ON980)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pENTR-H1 was obtained from Invitrogen; the H1 RNA gene promoter was a gift from Reuven Agami, NKI, Amsterdam, Netherlands; the Tet07 operator was a gift from Manfred Gossen, MDC, Berlin, Germany.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are some minor discrepancies between the depositor's assembled sequence and Addgene's quality control sequence. These differences should not affect the function of the plasmid.