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pGLTR-X-GFP
(Plasmid #58245)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58245 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHR-SIN-CSGW
  • Backbone manufacturer
    Adrian Trasher, UCL, London, UK
  • Total vector size (bp) 11300
  • Vector type
    Mammalian Expression, Lentiviral, RNAi ; GATEWAY destionation vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    attR1-Cm(R)-CcdB-attR2
  • Alt name
    GATEWAY DEST cassette
  • Species
    GATEWAY DEST cassette
  • Insert Size (bp)
    1580
  • Promoter no

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site blunted EcoRI (unknown if destroyed)
  • 3′ cloning site blunted EcoRI (unknown if destroyed)
  • 5′ sequencing primer n.a.
  • 3′ sequencing primer n.a.
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    TetR-NLS-T2A-GFP
  • Species
    repressor/GFP fusion
  • Insert Size (bp)
    700
  • Promoter SFFV
  • Tags / Fusion Proteins
    • TetR
    • NLS
    • T2A
    • GFP

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n.a.
  • 3′ sequencing primer n.a.
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

There are some minor discrepancies between the depositor's assembled sequence and Addgene's quality control sequence. These differences should not affect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGLTR-X-GFP was a gift from Stephan Geley (Addgene plasmid # 58245 ; http://n2t.net/addgene:58245 ; RRID:Addgene_58245)
  • For your References section:

    Development of a Multipurpose GATEWAY-Based Lentiviral Tetracycline-Regulated Conditional RNAi System (GLTR). Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S. PLoS One. 2014 May 19;9(5):e97764. doi: 10.1371/journal.pone.0097764. eCollection 2014. 10.1371/journal.pone.0097764 PubMed 24841113