PurposeGATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selection
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||58246||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 12000
Vector typeMammalian Expression, Lentiviral, RNAi ; GATEWAY destionation vector
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Insert Size (bp)600
- Promoter SFFV
/ Fusion Protein
- TetR-NLS-T2A (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n.a.
- 3′ sequencing primer n.a. (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
There are some minor discrepancies between the depositor's assembled sequence and Addgene's quality control sequence. These differences should not affect the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGLTR-X-PURO was a gift from Stephan Geley (Addgene plasmid # 58246 ; http://n2t.net/addgene:58246 ; RRID:Addgene_58246)
For your References section:Development of a Multipurpose GATEWAY-Based Lentiviral Tetracycline-Regulated Conditional RNAi System (GLTR). Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S. PLoS One. 2014 May 19;9(5):e97764. doi: 10.1371/journal.pone.0097764. eCollection 2014. 10.1371/journal.pone.0097764 PubMed 24841113