pcDNA3-WT-VLCAD-FLAG
(Plasmid #55823)

• Purpose: Expresses WT-VLCAD in mammalian cells
• Depositing Lab: Yoshimi Homma
• Publication: Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(1):C107-13. doi: 10.1152/ajpcell.00231.2009. Epub 2009 Nov 4.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Very long-chain specific acyl-CoA dehydrogenase
• Alt name: VLCAD
• Alt name: ACADVL
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Entrez Gene: ACADVL (a.k.a. ACAD6, LCACD, VLCAD)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: GAATTCATGCAGGCGGCTCGGATGGC
• 3′ sequencing primer: GCGGCCGCTCTGAAGCCAAGTGGGTTGCTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry