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pcDNA3-WT-VLCAD-FLAG
(Plasmid #55823)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 55823 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 55823-D50 50 μg of DNA in Tris buffer $495
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DNA 55823-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Very long-chain specific acyl-CoA dehydrogenase
  • Alt name
    VLCAD
  • Alt name
    ACADVL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Entrez Gene
    ACADVL (a.k.a. ACAD6, LCACD, VLCAD)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer GAATTCATGCAGGCGGCTCGGATGGC
  • 3′ sequencing primer GCGGCCGCTCTGAAGCCAAGTGGGTTGCTG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-WT-VLCAD-FLAG was a gift from Yoshimi Homma (Addgene plasmid # 55823 ; http://n2t.net/addgene:55823 ; RRID:Addgene_55823)

  • For your References section:

    Dysregulation of very long chain acyl-CoA dehydrogenase coupled with lipid peroxidation. Kabuyama Y, Suzuki T, Nakazawa N, Yamaki J, Homma MK, Homma Y. Am J Physiol Cell Physiol. 2010 Jan;298(1):C107-13. doi: 10.1152/ajpcell.00231.2009. Epub 2009 Nov 4. 10.1152/ajpcell.00231.2009 PubMed 19889959
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