S142D drebrin-YFP
(Plasmid
#58336)
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Purposemammalian expression of human drebrin-YFP phospho-mimetic mutant
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Depositing Lab
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Depositing OrganizationKing's College London
Located in United Kingdom of Great Britain and Northern Ireland -
Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58336 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 58336-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 58336-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEYFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedrebrin
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Alt namedrebrin E isoform
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SpeciesH. sapiens (human)
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MutationS142D, phospho-mimetic mutant
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Entrez GeneDBN1 (a.k.a. D0S117E)
- Promoter CMV
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Tag
/ Fusion Protein
- EYFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site SalI (destroyed during cloning)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 58336-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 58336-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
S142D drebrin-YFP was a gift from Phillip Gordon-Weeks (Addgene plasmid # 58336 ; http://n2t.net/addgene:58336 ; RRID:Addgene_58336) -
For your References section:
Drebrin contains a cryptic F-actin-bundling activity regulated by Cdk5 phosphorylation. Worth DC, Daly CN, Geraldo S, Oozeer F, Gordon-Weeks PR. J Cell Biol. 2013 Sep 2;202(5):793-806. doi: 10.1083/jcb.201303005. Epub 2013 Aug 26. 10.1083/jcb.201303005 PubMed 23979715