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FRP718_PACT1(-1-520)-LexA-ER-haB42-TCYC1
(Plasmid #58431)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58431 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS-derived
  • Backbone manufacturer
    Robert Gnuegge
  • Backbone size w/o insert (bp) 4396
  • Total vector size (bp) 7244
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LexA-ER-B42
  • Species
    Synthetic
  • Insert Size (bp)
    2848
  • Promoter Act1 promoter (-520;1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer AATTAACCCTCACTAAAGGG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For integration in yeast, linearize plasmid with PacI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRP718_PACT1(-1-520)-LexA-ER-haB42-TCYC1 was a gift from Joerg Stelling (Addgene plasmid # 58431 ; http://n2t.net/addgene:58431 ; RRID:Addgene_58431)
  • For your References section:

    Inducible, tightly regulated and growth condition-independent transcription factor in Saccharomyces cerevisiae. Ottoz DS, Rudolf F, Stelling J. Nucleic Acids Res. 2014 Jul 17. pii: gku616. 10.1093/nar/gku616 PubMed 25034689