VV020: WT Arch-3 - eGFP in pET28b
(Plasmid #58488)

• Purpose: Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coli
• Depositing Lab: Adam Cohen
• Publication: Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):5939-44. doi: 10.1073/pnas.1215595110. Epub 2013 Mar 25.

Backbone

• Vector backbone: pET28b
• Backbone size w/o insert (bp): 5269
• Total vector size (bp): 6772
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Express in BL21(DE3); See attached document or Maclaurin et al 2013 [PMID 23530193] for expression instructions.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Archaerhodopsin-3
• Alt name: Arch
• Species: Halorubrum sodomense
• Insert Size (bp): 1503
• GenBank ID: BAA09452.1
• Promoter: T7
• Tag / Fusion Protein:
  • eGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: TAA TAC GAC TCA CTA TAG GG
• 3′ sequencing primer: GCT AGT TAT TGC TCA GCG G

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We originally received this gene (Archaerhodopsin-3) from the Boyden Lab at MIT.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry