Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

VV020: WT Arch-3 - eGFP in pET28b
(Plasmid #58488)


Item Catalog # Description Quantity Price (USD)
Plasmid 58488 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5269
  • Total vector size (bp) 6772
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Express in BL21(DE3); See attached document or Maclaurin et al 2013 [PMID 23530193] for expression instructions.
  • Copy number


  • Gene/Insert name
  • Alt name
  • Species
    Halorubrum sodomense
  • Insert Size (bp)
  • GenBank ID
  • Promoter T7
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer TAA TAC GAC TCA CTA TAG GG
  • 3′ sequencing primer GCT AGT TAT TGC TCA GCG G
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We originally received this gene (Archaerhodopsin-3) from the Boyden Lab at MIT.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VV020: WT Arch-3 - eGFP in pET28b was a gift from Adam Cohen (Addgene plasmid # 58488 ; ; RRID:Addgene_58488)
  • For your References section:

    Mechanism of voltage-sensitive fluorescence in a microbial rhodopsin. Maclaurin D, Venkatachalam V, Lee H, Cohen AE. Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):5939-44. doi: 10.1073/pnas.1215595110. Epub 2013 Mar 25. 10.1073/pnas.1215595110 PubMed 23530193