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Purposehelper plasmid for bacterial conjugal DNA transfer
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Depositing Lab
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Depositing OrganizationMichigan State University
Located in United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58495 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 58495-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 58495-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepRL542
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)HB101
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameencodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
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Alt nameMobColK, M.AvaI, M.Eco47II
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
MobColK, M.AvaI, M.Eco47II
Please see attached map for more information on how this vector was constructed and cloning details.
DNA (Catalog # 58495-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 58495-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRL528 was a gift from Peter Wolk (Addgene plasmid # 58495 ; http://n2t.net/addgene:58495 ; RRID:Addgene_58495) -
For your References section:
Conjugal transfer of DNA to cyanobacteria. Elhai J, Wolk CP. Methods Enzymol. 1988;167:747-54. 0076-6879(88)67086-8 [pii] PubMed 3148842
Map uploaded by the depositor.
