|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||58495||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Growth in Bacteria
Gene/Insert nameencodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
Alt nameMobColK, M.AvaI, M.Eco47II
MobColK, M.AvaI, M.Eco47II
Please see attached map for more information on how this vector was constructed and cloning details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRL528 was a gift from Peter Wolk (Addgene plasmid # 58495 ; http://n2t.net/addgene:58495 ; RRID:Addgene_58495)
For your References section:Conjugal transfer of DNA to cyanobacteria. Elhai J, Wolk CP. Methods Enzymol. 1988;167:747-54. 0076-6879(88)67086-8 [pii] PubMed 3148842