pEBG Jip3 (nterm)
(Plasmid #58942)

• Purpose: interacts with MAPK signaling module
• Depositing Lab: Roger Davis
• Publication: Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

Backbone

• Vector backbone: pEBG
• Backbone size w/o insert (bp): 6000
• Total vector size (bp): 6810
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mitogen-activated protein kinase 8 interacting protein 3
• Alt name: Jip3
• Alt name: Mapk8ip3
• Species: M. musculus (mouse)
• Insert Size (bp): 810
• Mutation: deleted aa 271-1817
• GenBank ID: AF178636
• Entrez Gene: Mapk8ip3 (a.k.a. D17Wsu15e, JIP-3, JSAP1, JSAP1a, JSAP1b, JSAP1c, JSAP1d, Jip3, Syd2, mKIAA1066)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: Gex 5'
• 3′ sequencing primer: Gex 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry