pINDgw Cbx1 V5 EcoDam
(Plasmid
#59203)
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PurposeMammalian DamID positive control plasmid. Expresses Cbx1-V5-Dam.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 59203 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepINDgw RFA-V5-EcoDam
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Backbone manufacturervan Steensel lab, Addgene plasmid 59204
- Total vector size (bp) 6596
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Vector typeMammalian Expression ; DamID
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCbx1
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SpeciesM. musculus (mouse)
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Entrez GeneCbx1 (a.k.a. Cbx, Cbx-rs2, E430007M08Rik, HP1B, Hp1beta, M31, MOD1)
- Promoter heat shock minimal pomoter
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Tags
/ Fusion Proteins
- V5 (C terminal on backbone)
- Dam (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pCasper-hs
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pINDgw Cbx1 V5 EcoDam was a gift from Bas van Steensel (Addgene plasmid # 59203 ; http://n2t.net/addgene:59203 ; RRID:Addgene_59203) -
For your References section:
Human heterochromatin proteins form large domains containing KRAB-ZNF genes. Vogel MJ, Guelen L, de Wit E, Peric-Hupkes D, Loden M, Talhout W, Feenstra M, Abbas B, Classen AK, van Steensel B. Genome Res. 2006 Dec;16(12):1493-504. Epub 2006 Oct 12. 10.1101/gr.5391806 PubMed 17038565
Map uploaded by Addgene staff.
Map uploaded by the depositor.
