pINDgw RFA-V5-EcoDam
(Plasmid #59204)

• Purpose: (Empty Backbone) Mammalian DamID vector for fusing gene of interest with Dam-V5 using Gateway cloning
• Depositing Lab: Bas van Steensel
• Publication: Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Epub 2006 Oct 12.

Backbone

• Vector backbone: pIND(V5)EcoDam
• Backbone manufacturer: van Steensal lab, Addgene plasmid 59201
• Backbone size (bp): 7660
• Modifications to backbone: added Gatweway cassette
• Vector type: Mammalian Expression ; DamID
• Promoter: Heat Shock Minimal Promoter
• Selectable markers: Neomycin (select with G418)
• Tags / Fusion Proteins:
  • V5 (C terminal on backbone)
  • Dam (DNA adenine methyltransferase) (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Unknown

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pCasper-hs (GCAACTACTGAAATCTGCCAAG)
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that this vector can not be used to express Dam only.