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pCAG-HA-NLGN4 WT
(Plasmid #59320)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59320 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4835
  • Total vector size (bp) 7265
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLGN4
  • Alt name
    Neuroligin 4
  • Alt name
    NLGN4X
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2430
  • GenBank ID
    BC034018
  • Entrez Gene
    NLGN4X (a.k.a. ASPGX2, AUTSX2, HLNX, HNL4X, NLGN4)
  • Promoter CAG
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
  • 3′ sequencing primer Bglob-pA-R (5'-TTTTGGCAGAGGGAAAAAGA-3'); pCAG_rev_seq (5'-CAGCCACCACCTTCTGATAG-3')
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See map & sequence file for insert-releasing enzymes (e.g. NheI / XhoI).
HA-epitope tag was introduced at the mature N-terminus after the signal sequence

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-HA-NLGN4 WT was a gift from Peter Scheiffele (Addgene plasmid # 59320 ; http://n2t.net/addgene:59320 ; RRID:Addgene_59320)
  • For your References section:

    Disorder-associated mutations lead to functional inactivation of neuroligins. Chih B, Afridi SK, Clark L, Scheiffele P. Hum Mol Genet. 2004 Jul 15;13(14):1471-7. Epub 2004 May 18. 10.1093/hmg/ddh158 PubMed 15150161