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pBrain-GFP-TACC3KDP(S558A)-shTACC3
(Plasmid #59357)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59357 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBrain
  • Backbone manufacturer
    Stephen J Royle
  • Backbone size w/o insert (bp) 5438
  • Total vector size (bp) 7952
  • Modifications to backbone
    Addition of N-terminal GFP tag. shRNA to knock down endogenous TACC3.
  • Vector type
    Mammalian Expression, RNAi
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Transforming Acidic Coiled Coil protein 3
  • Alt name
    TACC3
  • Alt name
    ERIC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2514
  • Mutation
    Silent mutations in amino acids 30-33 to confer siRNA resistance. Serine 558 changed to Alanine.
  • GenBank ID
    NM_006342.2
  • Entrez Gene
    TACC3 (a.k.a. ERIC-1, ERIC1, Tacc4, maskin)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    shRNA sequence as referenced in Gergely, 2003.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBrain-GFP-TACC3KDP(S558A)-shTACC3 was a gift from Stephen Royle (Addgene plasmid # 59357 ; http://n2t.net/addgene:59357 ; RRID:Addgene_59357)
  • For your References section:

    A TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by inter-microtubule bridging. Booth DG, Hood FE, Prior IA, Royle SJ. EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 2011 Feb 4. 10.1038/emboj.2011.15 PubMed 21297582