pUltra-Smurf-luc-RTTA3
(Plasmid #59438)

• Purpose: silence doxycyclin inducible pUltra-dox (also expresses luciferase)
• Depositing Labs: Yildirim Dogan, Kitai Kim
• Publication: pUltra plasmids (unpublished)

Backbone

• Vector backbone: pUltra-Chili
• Backbone manufacturer: Moore Lab, Addgene plasmid 48687
• Modifications to backbone: dtomato was replaced with AmCyam
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: luciferase

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: NA

Gene/Insert 2

• Gene/Insert name: Reverse TET-Transactivator 3
• Alt name: RTTA3
• Mutation: loxP5171

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: Luc-F
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

loxP5171 at positions after cppt and before WPRE

Identical to pUltra-puro-luc-RTTA3 (Addgene plasmid 58764) but puro is replaced by AmCyan, for FACS Sorting instead of puro selection.