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pGS1021
(Plasmid #59575)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59575 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    Fermentas/Thermo
  • Backbone size w/o insert (bp) 2710
  • Total vector size (bp) 3824
  • Vector type
    E. coli cloning vector
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Twin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
  • Species
    Synthetic
  • Insert Size (bp)
    1171
  • Promoter None
  • Tag / Fusion Protein
    • Twin-Strep tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site SphI (not destroyed)
  • 5′ sequencing primer AGG GTT TTC CCA GTC ACG ACG TT
  • 3′ sequencing primer GAG CGG ATA ACA ATT TCA CAC AGG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Twin-Strep tag sequence was amplified from a commercial plasmid pPRIBA101 (IBA, Goettingen, Germany) and mutated by site-directed mutagenesis to an improved sequence for PCR amplification of the tagging cassette. The encoded amino acids were not changed by this mutagenesis.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGS1021 was a gift from Monika Golas & Bjoern Sander (Addgene plasmid # 59575 ; http://n2t.net/addgene:59575 ; RRID:Addgene_59575)
  • For your References section:

    Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by Homologous Recombination and Characterization of Endogenous Macromolecular Assemblies in Saccharomyces cerevisiae. Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard JR, Golas MM, Sander B. Mol Biotechnol. 2014 Jun 27. 10.1007/s12033-014-9778-5 PubMed 24969434