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pQL269
(Plasmid #59802)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59802 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pINTts
  • Backbone manufacturer
    Haldimann,A. and Wanner,B.L.
  • Backbone size (bp) 2000
  • Modifications to backbone
    The pQL269 plasmid (lac–cre,SpR, and ori Ts) was constructed by ligating the EcoRI–PvuII fragment from pQL114, containing aadA (conferring spectinomycin resistance) and the lac–cre chimeric gene, to a BglI(blunt)– EcoRI fragment containing the temperature-senstive replication origin from pINT-ts.
  • Vector type
    Bacterial Expression, Cre/Lox
  • Promoter lambdaPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    BUN14
  • Growth instructions
    Temperature sensitivity strain; grow at 42
  • Copy number
    Unknown

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQL269 was a gift from Stephen Elledge (Addgene plasmid # 59802 ; http://n2t.net/addgene:59802 ; RRID:Addgene_59802)
  • For your References section:

    The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes. Liu Q, Li MZ, Leibham D, Cortez D, Elledge SJ. Curr Biol. 1998 Dec 3;8(24):1300-9. S0960-9822(07)00560-X [pii] PubMed 9843682