Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pDEST26 Ago2 S387A
(Plasmid #59905)


Item Catalog # Description Quantity Price (USD)
Plasmid 59905 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pDEST 26
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 7481
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy


  • Gene/Insert name
    Argonaute 2
  • Alt name
    Protein Slicer
  • Alt name
    EIF2C 2
  • Species
    H. sapiens (human)
  • Mutation
    S387A, phospho-null alanine
  • Entrez Gene
    AGO2 (a.k.a. CASC7, EIF2C2, LINC00980, PPD, Q10)
  • Promoter CMV
  • Tag / Fusion Protein
    • 6xHis (N-termi tag) (N terminal on backbone)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST26 Ago2 S387A was a gift from Carl Novina (Addgene plasmid # 59905 ; ; RRID:Addgene_59905)
  • For your References section:

    Akt-mediated phosphorylation of argonaute 2 downregulates cleavage and upregulates translational repression of MicroRNA targets. Horman SR, Janas MM, Litterst C, Wang B, MacRae IJ, Sever MJ, Morrissey DV, Graves P, Luo B, Umesalma S, Qi HH, Miraglia LJ, Novina CD, Orth AP. Mol Cell. 2013 May 9;50(3):356-67. doi: 10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18. 10.1016/j.molcel.2013.03.015 PubMed 23603119